Heat-Resistant Properties of α-Chymotrypsin Adsorbed onto Biomass Charcoal Powder

Biomass charcoal powder (BCP) was used as a carrier for immobilization of α-chymotrypsin through adsorption. BCP was derived from plant biomass wastes such as dumped bamboos by oxygen-free pyrolysis at low temperatures and grinding with a jet mill. The activity of adsorbed α-chymotrypsin was strongly dependent upon the kind of BCP. The thermal denaturation curve of adsorbed α-chymotrypsin was shifted to high temperature, compared to that of free one. When α-chymotrypsin adsorbed onto BCP of bamboos was incubated at 45 ̊C, the half-life of adsorbed α-chymotrypsin was 2.6 times greater than that of free one. After incubation at 45 ̊C, the remaining activity of adsorbed α-chymotrypsin markedly depended on the kind of BCP.